Coding

Part:BBa_K2636000:Design

Designed by: Sandro Papais   Group: iGEM18_Bordeaux   (2018-10-09)


Aldehyde dehydrogenase 1 ( Aldh1 )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 319
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 237
    Illegal NgoMIV site found at 555
    Illegal NgoMIV site found at 646
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 598


Design Notes

Codon optimisation for Escherichia coli. Moreover, three illegal restriction sites were replaced :
- In position 416-423 ( NotI ), GGC Gly ( 418-420 ) was replaced by GGA Gly.
- In position 997-1002 ( PstI ), CTG Leu ( 997-999 ) was replaced by CTT Leu and 1348-1353 ( PstI ), CTG Leu ( 1348-1350 ) was replaced by CTT Leu.

Source

Raoutella ornithinolytica BF6

References

Hossain, G. S., Yuan, H., Li, J., Shin, H., Wang, M., Du, G., … Liu, L. (2017). crossm Metabolic Engineering of Raoultella, 83(1), 1–15.